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. 2019 Jan 24;9:736. doi: 10.1038/s41598-018-36859-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Morphology and differentiation status of mutant cells. (a) Morphological features of keratinocytes and fibroblasts were assessed by phase contrast microscopy, Scale bar: 50 μm. (b,c) The clonogenic capacity of mutant and wild type keratinocytes was determined by measuring absorbance at 400–700 nm of rhodanile blue-stained cells. (d,e) Terminal differentiation markers of keratinocytes KRT1 and IVL were analyzed in both wild type and mutant cells by flow cytometry. Histograms show the mean ± SD of at least three independent experiments.