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. 2019 Jan 24;9:736. doi: 10.1038/s41598-018-36859-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Morphological features of patient’s skin. (a) Skin sections from the patient (carrying an ADNP mutant allele) and an age-matched healthy control (ADNP wt) were labelled with antibodies against CCNA and KRT10 markers to assess their expression and distribution along epidermal layers. Nuclei were counterstained with DAPI. (b) More than 100 cells for both patient and control samples were counted using ImageJ software and the percentage of cells expressing CCNA was determined. (c) Nuclear distribution of phospho-histone H3 that marks chromatin condensation changes during the cell cycle. (d) The punctate staining of phospho-histone H3 associated with chromatin condensation (CCpH3 + cells) was determined in at least 100 cells of both wild type and mutant genotypes. Scale bar in all immunofluorescence images: 500 μm. Histogram shows the mean ± SD of at least three independent experiments.