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. 2019 Jan 24;9:564. doi: 10.1038/s41598-018-37252-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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WJ-MSCs stored in AD CSF maintain viability. WJ-MSCs stored under hypothermic conditions (one MEMα 1x media group, four AD CSF samples, and three normal CSF samples) were stained with Annexin V/7-AAD and subsequently analyzed by fluorescence-activated cell-sorting (FACS). Images of the cells were taken, and CCK-8 assays were performed. (a) Apoptosis was evaluated after 72 hours. (b) Ratio of non-apoptotic/early apoptotic cells from FACS analysis. (c) Images of seeded cells were taken (scale bar = 40 μm). (d) CCK-8 assay results acquired every 24 hours up to 72 hours. ^*P < 0.05; ^***P < 0.001.