Skip to main content
. 2019 Jan 24;87(2):e00604-18. doi: 10.1128/IAI.00604-18

FIG 6.

FIG 6

IgG, IgM, CRP, and C3 opsonization and survival of CWZ_016 and the CWZ_016 licA mutant in IgA/IgM-deficient serum with and without IgM supplementation. (A to D) NTHi bacteria were incubated with 10% IgA/IgM-deficient serum supplemented with or without 10 µg/ml IgM (C3) or with 10% HI-IgA/IgM-deficient serum supplemented with or without 10 µg/ml IgM (IgG, IgM, and CRP), and surface binding of IgG (A), IgM (B), CRP (C), or C3 (D) was determined by flow cytometry (n = 3). (E) Bacteria were incubated with 10% HI-IgA/IgM-deficient serum, 10% IgA/IgM-deficient serum, or 10% IgA/IgM-deficient serum supplemented with 10 µg/ml IgM for 1 h, and survival was determined by dividing the CFU in 10% IgA/IgM-deficient serum with or without IgM by the CFU in 10% HI-IgA/IgM-deficient serum after 1 h of incubation (n = 4). A two-tailed paired t test was used for statistical analysis. *, P < 0.05; **, P < 0.01; NS, not significant.