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. 2018 Dec 21;8(1):325–336. doi: 10.1002/cam4.1923

Figure 2.

Figure 2

LRP8 is required for the survival of breast cancer cells. Breast cancer cell lines were transfected with one of the two siRNAs against LRP8 (#2 and #3: orange and red, respectively) or with a control siRNA (black). A, LRP8 protein levels were evaluated by Western blotting 144 h after transfection. Actin was used as a loading control. One experiment of at least two (three for MDA‐MB‐468, HCC70, HCC38, BT‐20, and MCF7), all giving similar results, is shown. B, Cell viability was assessed in MTT or WST‐1 assays, 144 h after transfection. Results are presented as percent cell viability relative to cells treated with control siRNA (100%). Data are the means + SD of at least three independent experiments. C, Dynamic monitoring of TNBC cell adhesion and proliferation with the xCELLigence system. Cell proliferation in response to siRNA treatment was monitored for 144 h (x‐axis), by evaluating cell index (y‐axis) values with xCELLigence System. The cell index was normalized relative to its value at the time of the transfection (cell index = 1 at t = 0)