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Primer sequences for RT‐PCR
| Gene | Primers sequence (5′‐3′) | Annealing T (°C) |
|---|---|---|
| CCNG1‐F | AATGAAGGTACAGCCCAAGCA | 63 |
| CCNG1‐R | GCTTTGACTTTCCAACACACC | |
| β‐actin‐F | GAGGCACTCTTCCAGCCTTC | 55 |
| β‐actin‐R | GGATGTCCACGTCACATTC | |
| Notch3‐F | TCTCAGACTGGTCCGAATCCAC | 57 |
| Notch3‐R | CCAAGATCTAAGAACTGACGAGCG | |
| P53‐F | TGAAGTCTCATGGAAGCCAGC | 54 |
| P53‐R | GCTCTTTTTCACCCATCTACAG |
