Figure 3.

Mean ± SEM of relative expression (measured as percentage of immunopositive area) of MMP2 and MMP9 in ovaries of control and prenatal T-treated fetal D90 and D140 and adult 21-mo-sheep. Relative expression levels were measured in granulosa cells from primary, primordial, small and large preantral, and antral follicles and stroma cells. Note scale bar difference between follicular and stromal compartments. ##Large effect as determined by Cohen effect size analysis. ND indicates not detectable; SEM indicates standard error of the mean; MMP, matrix metalloproteinase; D90, fetal day 90; D140, fetal day 140.