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RNA library preparation procedure for sequencing RNA. The mRNA molecule is fragmented. Adapter molecules with known sequences are ligated to the 3′ and 5′ end of the fragmented mRNA molecules. Ligating adapters with unique barcode sequences to fragmented mRNA molecules mediates processing full-length mRNA sequence. mRNA = messenger RNA; RNA = ribonucleic acid. Source: Reprinted with permission from Hrdlickova, Toloue, and Tian (2017). Copyright 2016 by Wiley Periodicals.
