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Simulated single-Trp (A) and double-Trp (B) hopping advantages for constructs modeled on Re124WCuI (d(Re–Cu) = 20 Å) and Re126WWCuI (d(Re–Cu) = 23 Å). Optimum positioning of the intervening Trp in the Re124WCuI construct (r1 = 7 Å, r2 = 13 Å, colinear) leads to a predicted 103.8 hopping advantage. In the Re126WWCuI model, optimum positioning of the two Trp residues (r1 = 6 Å, r2 = 7 Å, r3 = 10 Å, colinear) produces a 105.5 hopping advantage over single-step tunneling.
