Fig. 2. (a) Chemical structure of MNG-6,14 is shown to illustrate anomeric protons (H_a), their couplings with the neighbouring protons, and a set of protons of interest responsible for key NOE correlation signals. (b) Partial ¹H NMR spectrum in the anomeric region for MNG-6,14 showing its high anomeric purity. The spectrum of MNG-6,14 showed a doublet at 4.35 ppm, with a vicinal axial–axial coupling constant (³J_aa) of 8.0 Hz, a typical peak characteristic of β-anomeric proton (H_a). The peaks for the anomeric protons with α-linkage (H_e) appeared at ∼5.15 ppm with a vicinal axial–equatorial coupling constant (³J_ae) of 4.0 Hz. (c) Partial 2D NOESY NMR spectrum of MNG-6,14 in CD₃OD. Only key ¹H–¹H NOE correlation signals are assigned. MNG-6,14 showed an NOE correlation signal between two protons of H_a and H₁. Because of a close proximity in space, an NOE correlation signal between H₁ and H₃ was additionally observed in the spectrum.