Fig. 5.

miR-342-3p targets FOXQ1 in NPC cells. a and b Western blot analysis of indicated proteins in C666 and CNE2 cells transfected with indicated constructs. Numbers represent fold change in protein levels. Data represent mean of three independent experiments. c Luciferase reporter assay performed in C666 cells demonstrated that the reporter construct harboring wild-type (wt) FOXQ1 3’-UTR but not the mutant (mt) was suppressed by overexpression of miR-342-3p. ^*P < 0.05. N.S. indicates no significance. d Determination of the relationship between miR-342-3p and FOXQ1 protein expression in NPC specimens (n = 79) by Pearson’s correlation coefficient analysis. e Western blot analysis of FOXQ1 protein levels in indicated cells