Fig. 2.

Stimulation of in vitro dual incision reactions with CPD substrates by the presence of mismatched bases or an AP site. Internally ³²P-labeled DNA substrates containing a site-specific CPD (~ 200 bp in length) were incubated with six purified recombinant NER factors, and excised oligonucleotides containing both the ³²P label and CPD were detected by denaturing polyacrylamide gel electrophoresis and the following autoradiography as described previously [12, 50]