Fig. 3.

Kinetic analyses of XPC recruitment to DNA damage sites in living cells. DNA damage was induced with the 780-nm femtosecond laser and three-photon absorption within subnuclear regions of human osteosarcoma U2OS cells stably expressing mCherry-fused XPC. Cells were pre-treated either with siRNA targeting DDB2 (a) or with a histone deacetylase inhibitor, trichostatin A (TSA) (b). Time-lapse images were acquired every 5 s with the Olympus FV-3000 confocal laser scanning microscope, and relative fluorescence intensities of the irradiated areas were quantified. Mean values and standard deviations were calculated from 10 (a) and 17 (b) samples, respectively