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. 2019 Jan 24;19:8. doi: 10.1186/s12896-019-0499-5

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Influence of rising nisin levels and temperature 30 °C (panel a), growing nisin levels and temperature 20 °C (panel b), and induction at OD₆₀₀ (panel c) at various time points on the expression of rE7 proteins in L. lactis containing pNZ8123-HPV16-optiE7 (Left) and pNZ8123-HPV16-E7 (right). Panel a and b: Induction time with 1 ng/mL (diamond), 5 ng/mL (square), 10 ng/mL (triangles), 15 ng/mL (multiple), and 20 ng/mL (star) nisin. Panel c (at 20 °C): induction at OD₆₀₀ = 0.5 (diamond), OD₆₀₀ = 0.6 (square), OD₆₀₀ = 0.7 (triangle), OD₆₀₀ = 0.8 (multiple)