Table 5.
List of genes coding for enzymes identified in 3S polluted system involved in hydrocarbon degradation
| Enzyme | Enzyme function | Bit score | e value |
|---|---|---|---|
| Rieske non-heme iron oxygenase (RO) | Involved in regio- and stereoselective transformation of a wide array of aromatic hydrocarbons | 86.12 | 6.87e−24 |
| Rieske non-heme iron oxygenase (RO), α-subunit | The catalytic domain of RO, aromatic ring-hydroxylating dioxygenases | 49.89 | 1.67e− 09 |
| Vanillate O-demethylase oxygenase subunit | Catalyzes the reductive conversion of vanillate into protocatechuate and formaldehyde | 43.12 | 7.72e− 07 |
| Dicamba O-demethylase oxygenase | Catalyzes the conversion of dicamba (2-methoxy-3,6-dichlorobenzoic acid) to DCSA (3,6-dichlorosalicylic acid) | 43.12 | 7.72e− 07 |
| Phenylpropionate dioxygenase, large terminal subunit | Catalyse the Insertion of both atoms of molecular oxygen into positions 2 and 3 of the phenyl ring of phenylpropionate, yielding cis-3-(3-carboxyethyl)-3,5-cyclohexadiene-1,2-diol | 44.41 | 8.48e− 07 |
| Rieske non-heme iron oxygenase (RO), ferredoxin component of biphenyl dioxygenase (BPDO) and carbazole 1,9a-dioxygenase (CARDO) | BPDO degrades biphenyls and polychlorinated biphenyls. CARDO catalyzes angular dioxygenation of carbazole at the C1 and C9a positions | 41.32 | 2.47e− 06 |
| 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) | Catechol pathway | 51.59 | 1.89e− 09 |
| 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase, C-terminal subunit | Decarboxylate OPET to HHDD; isomerize HHDD to OHED (4-hydroxyphenylacetate degradation) | 45.57 | 1.98e− 07 |
| 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase | Catalyzes the NAD+-dependent oxidation of 2,3-dihydro-2,3-dihydroxybenzoate to produce an aromatic compound 2,3-didihydroxybenzoic acid | 60.76 | 1.88e− 12 |
| 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase | Catalyze the anaerobic degradation of benzoyl-CoA to 3-hydroxypimeloyl-CoA | 57.24 | 3.92e− 11 |
| 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase (DHB-DH) | Catalyzes the NAD-dependent conversion of 1,2-dihydroxycyclohexa-3,4-diene carboxylate to a catechol | 56.77 | 6.19e− 11 |
| cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase (BphB) | Biphenyl/polychlorinated biphenyl degradation pathway | 40.03 | 6.01e− 05 |
| Cyclohexanol reductase | Catalyse the reversible oxidoreduction of hydroxycyclohexanone derivatives | 55.99 | 1.23e− 10 |
| Cyclohexanecarboxyl-CoA dehydrogenase | Funnel the alicyclic acid cyclohexane carboxylate into the anaerobic benzoate degradation pathway | 45.68 | 2.44e− 07 |
| Dibenzothiophene (DBT) desulfurization enzyme C; DszC | Converts DBT to DBT-sulfoxide, which is then converted to DBT-sulfone | 33.07 | 6.14e− 03 |
| 3-Oxoadipyl-CoA thiolase | Participates in benzoate degradation via hydroxylation | 105.64 | 6.67e− 28 |
| Acetyl-CoA acetyltransferases | Fatty acid degradation, benzoate degradation, ethylbenzene degradation | 153.92 | 2.60e− 46 |
| C-terminal substrate binding domain of LysR-type transcriptional regulators involved in the catabolism of aromatic compounds | Involved in degradation of aromatic compounds | 80.63 | 1.43e− 20 |
| C-terminal substrate binding domain of LysR-type transcription regulators, BenM, CatM, and CatR | Participate in benzoate degradation | 48.76 | 1.71e− 08 |
| C-terminal substrate binding domain of LysR-type transcriptional regulators that involved in the catabolism of nitroaromatic/naphthalene compounds | Involved in the degradation of dinitrotoluene and similar compounds | 43.74 | 1.06e− 06 |
| C-terminal substrate binding domain of LysR-type transcriptional regulators CbnR, ClcR and TfdR | Participate in the regulation of chlorocatechol catabolism | 39.19 | 3.95e− 05 |
| pca operon transcription factor PcaQ | Catabolism of protocatechuate | 35.46 | 1.09e− 03 |