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. 2019 Jan 18;9:792. doi: 10.3389/fendo.2018.00792

Figure 6.

Figure 6

Glucagon secretion and cell content are regulated by a subset of interactome proteins. (A) Glucagon secretion and (B) cell content in the context of 25 mM glucose, and (C) glucagon secretion and (D) cell content in the context of 5.5 mM glucose were assessed after siRNA-mediated gene silencing of the following proteins: Alpha-tubulin 2 (AT2), ATP synthase F1 subunit alpha (ATP5F1A), Malate dehydrogenase 1 (MDH1), Protein disulfide-isomerase (PDI), ELKS/Rab6-interacting/CAST family member 1 (ERC1), Aconitate hydratase mitochondrial (ACO2), Peroxiredoxin-2 (PRDX2), 14-3-3 protein zeta/delta (KCIP-1), FXYD domain-containing ion transport regulator 2 (FXYD2), histone H4, and GRP78 using pre-designed siRNAs for the mouse genome. After siRNA transfection, α-TC1-6 cells were cultured in DMEM containing 25 or 5.5 mM glucose for 24 h and glucagon levels were measured using ELISA. Values are expressed as mean ± SD (α = 0.05; n = 3–4). *p < 0.05; **p < 0.01; ***p < 0.001.