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. 2019 Jan 20;8(1):1565885. doi: 10.1080/20013078.2019.1565885

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Characterization of EVs secreted from HASCs. (a) Schematic representations of EV isolation from HASCs and the EV treatment of UVB-induced skin ageing. (b) Nanoparticle tracking analysis (NTA) showing the concentration of the EVs. (c) Classic and (d) cryogenic transmission electron microscope (TEM) pictures of EVs. The scale bars represent 100 nm (white) and 20 nm (yellow). (e) Western blot analysis of EVs. Expression of EV markers (TSG101, CD9, CD63 and CD81) and internal protein markers (GM130 and Calnexin).