Figure 5.
The effects of HASC-derived EVs on the wound recovery of UVB-irradiated HDFs. HDFs were seeded in 24-well plates at 1 × 105 cells per well and maintained in growth medium until they reached 90% confluence. The HDFs were then exposed to UVB radiation at a dose of 0.05 J/cm2 once a day for 3 days. The normal and UVB-irradiated HDFs were scratch-wounded with a sterilized micropipette tip and incubated in growth medium (GM), serum-free medium (SFM), BSA 0.1% and EVs (1 × 108 particles/mL) containing SFM for 24 h. (a, b) Light microscopic images of HDFs in the scratched area. The yellow dotted lines indicate the original wound edge in the scratch array. Scale bars represent 200 μm. (c) Wound recovery rates of HDFs into the scratched area. The wound recovery rate is presented as the percentage of scratch closure. The data are shown as the means ± standard deviations (n = 5).