Figure 6.

The effects of HASC-derived EVs on the (a) migration and (b) proliferation of UVB-irradiated HDFs. HDFs were exposed to UVB radiation at a dose of 0.05 J/cm² once a day for 3 days. (a) The normal and UVB-irradiated HDFs were seeded into the upper side of the transwell membrane and maintained in growth medium (GM), serum-free medium (SFM), BSA 0.1% and EVs (1 × 10⁸ particles/mL) containing SFM for 24 h. Migrated cells to the lower side were fixed with 4% paraformaldehyde and stained with crystal violet. Scale bars represent 100 μm. (b) The normal and UVB-irradiated HDFs were seeded in 24-well plates and maintained in growth medium (GM), serum-free medium (SFM), BSA 0.1% and EVs (1 × 10⁸ particles/mL) containing SFM for 48 h. The proliferation of HDFs was determined by CCK-8 assay. The data are shown as the means ± standard deviations (n = 4) with significance at *p < 0.01.