Figure 4.
PCA analysis of spectral variations during neutrophilic differentiation. (A,B) PC 1 and PC 2 of PCA of individual 32D cells during the 14- day differentiation identify significant sources of spectral variance over time. (C) PCA demonstrated spectral trends as cells progressed through neutrophilic differentiation states (myeloblast, promyelocytes, metamyelocytes, band cells, neutrophils). (D) Average PC 1 scores correlated with the level of cell maturation. *p < 0.05, **p < 0.01, ***p < 0.001.