Table 1. List of convergent and divergent primers used for circRNAs qRT-PCR validation.
Primers | Sequences (5′–3′) | Size (bp) |
---|---|---|
GAPDH CON-F GAPDH CON-R |
AGCCGTAACTTCTGTGCTGT TTCCCGTTCTCTGCCTTGAC |
234 |
GAPDH DIV-F GAPDH DIV-R |
ATGGTCCACATGGCCTCC CATCTTGTCTCAGGGATGC |
334 |
circRNA1001 CON-F circRNA1001 CON-R |
CCACTCAGTTCCCTGCTGAT TCTTTACTTTGTGGCTGGCTC |
82 |
circRNA1001 DIV-F circRNA1001 DIV-R |
TAGACAGCTCTGACAGCATGG TGCCACATGACTCATTAATTTC |
101 |
circRNA1684 CON-F circRNA1684 CON-R |
GGAGTCAACCTCACCACTGA CCCCGGTCATAGCACACAA |
71 |
circRNA1684 DIV-F circRNA1684 DIV-R |
CGATTACTCCATGTACCAGGCA GCACACAAACCTGTAATCCTGG |
155 |
circRNA3127 CON-F circRNA3127 CON-R |
AGGACCCTCATCCCTCGTTA GTCCACGGTGATGGATGAGTT |
114 |
circRNA3127 DIV-F circRNA3127 DIV-R |
TCACCCTCAACTACCTCAGGCT GTCACCCCTCCTTCAAACACAG |
78 |
circRNA7880 CON-F circRNA7880 CON-R |
AAAAGAAGCCGTCTCGGACA CCAGACGTTTTCTGGGGCTA |
132 |
circRNA7880 DIV-F circRNA7880 DIV-R |
AATCAGATAGCCACCATCTTG TGTAGCCTGTGACTGGGAAC |
85 |
circRNA998 DIV-F circRNA998 DIV-R |
GACGACCTGATGGATTATCACC TGCCATAATCTTGTTGGAATCA |
89 |
circRNA1000 DIV-F circRNA1000 DIV-R |
TCGGAAACAACTGAACTTATGA TGTTCTTCACTTATACCCTCTGG |
125 |
circRNA4287 DIV-F circRNA4287 DIV-R |
GTGTGAAAATAACGTGAAGGAA CTTCTAATTTCCTCACTCTCAGA |
102 |
circRNA5112 DIV-F circRNA5112 DIV-R |
GGCTAAGCAATTCTCGGTTGG TTGTAGCCTGTGACTGGGAACG |
77 |
circRNA8565 DIV-F circRNA8565 DIV-R |
GCTACTTCCAGCTGCAGATGTG ACACTGAGAACTTCAGGAACGC |
137 |