Fig. 2.

Treatment of respiring mitochondria with sodium azide has no effect on glutathione metabolism following exposure to H₂O₂. The concentrations of reduced (GSH) and oxidized (GSSG) glutathione were measured at indicated times following the addition of 25 μM H₂O₂ to isolated cardiac mitochondria (0.5 mg/ml) respiring on palmitoyl-carnitine (25 μM) and malate (100 μM). GSH (A) and GSSG (B) concentrations in the absence (black circles) or presence of sodium azide (black squares) or iodoacetamide (gray triangles). Values are presented as the mean ± SD (n = 3).