Table 2.

Primers sequence and PCR conditions for bsh and baiCD qPCR.

Group	Primer Sequence (5’-3’)	F/R	Cycling Conditions	Expected Product Size (bp)
1a	CACATATTGTGGCACGAACAATHGAR TGGGG	F	95°C for 10 min, (95°C for 15 sec, 55°C for 1 min) × 40 cycles	570
	CTGTGCCCGGATACAGATTAACRTAR TTRTT	R
1b	CGGCGTTCCGCATTTYTAYGARAA	F	95°C for 10 min, (95°C for 15 sec, 55°C for 1 min) × 40 cycles	318
	GTTCAATGCCAATCGGAATATCRAAR TTRTT	R
3c/e	TTTTGGCCGAACACTGGAYTAYGARTT	F	95°C for 5 min, (95°C for 15 sec, 54°C for 30 sec, 72 for 10 min) × 40 cycles	774
	TCAACGGAGCCCAGAATATGRAARA AYTG	R
baiCD	GGWTTCAGCCCRCAGATGTTCTTTG	F	94°C for 2 min, (94°C for 20 sec, 52°C for 30 sec, 69°C for 90 sec) × 35 cycles, 68°C for 10 min	1300
	GAATTCCGGGTTCATGAACATTCTKCKAAG	R