Valerate inhibits C difficile vegetative growth in batch
cultures. Vegetative cells were inoculated into supplemented brain heart
infusion broth containing varying concentrations of valerate (0, 1, 2, 3, 4, 5,
10, and 20 mM) and OD600 measurements were taken at 0, 2, 4, 6, and 8
hours. The change in OD600 (from a time point during the exponential
phase) was plotted against the concentrations of valerate tested.
(A)
C difficile ribotype 010, (B)
C difficile ribotype 012, (C)
C difficile ribotype 027, (D)
B. uniformis, (E)
B. vulgatus,
(F)
C. scindens. Error bars represent the mean ± standard
deviation, * p<0.05, ** p<0.01,
*** p<0.001.