Skip to main content
. Author manuscript; available in PMC: 2019 Oct 4.
Published in final edited form as: Mol Cell. 2018 Sep 20;72(1):127–139.e8. doi: 10.1016/j.molcel.2018.08.016

Figure 5. The assembly of Brca1/Bard1 heterodimers onto stalled replication forks is defective in Bard1KA/KA and Bard1SF/SF cells.

Figure 5.

A) For iPOND analysis (Sirbu et al., 2012), cell lysates and iPOND-purified fractions were prepared from untreated cell cultures and from parallel cultures pulsed-labeled for 10 minutes with IdU. The IdU-labeled cultures were harvested immediately or after a subsequent 90-chase with 2mM hydroxyurea (HU) and/or 100 nM olaparib (PARPi).

B) Immunoblot analysis of protein abundance in the input cell lysates (left) and the corresponding iPOND-purified fractions (right) from Bard1+/+ and Bard1SF/SF cells.

C) Immunoblot analysis of protein abundance in the input cell lysates (left) and the corresponding iPOND-purified fractions (right) from Bard1+/+ and Bard1KA/KA cells.