Table 1. Physiological parameters (protocol 1).
Bederson score | Weight(gram) | Temperature(°C) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Day of injection | PBS | MNCs | RACs | PBS | MNCs | RACs | PBS | MNCs | RACs | |
Day 1 |
Pre-operation | 15±0 | 15±0 | 15±0 | 23.95±1.60 | 23.53±1.58 | 24.45±1.52 | 31.52±8.46 | 30.27±9.07 | 32.70±7.45 |
Injection | 11.9 ±2.0 |
11.5 ±2.5 |
12.8 ±1.8 |
27.82±8.29 | 28.15±8.37 | 26.68±7.14 | 31.75±7.27 | 31.24±7.79 | 33.77±6.17 | |
Sacrificed | 14.3±1.1 | 14.3±0.9 | 14.8±0.4 | 28.20±7.48 | 29.62±6.82 | 28.28±6.08 | ||||
Day 3 |
Pre-operation | 15±0 | 15±0 | 15±0 | 24.54±1.72 | 24.84±1.12 | 24.63±0.99 | 23.38±1.74 | 23.50±1.39 | 23.03±1.16 |
Injection | 13 ±1.2 |
12.3 ±1.9 |
13.4 ±0.8 |
37.83±0.69 | 37.76±0.40 | 37.80±0.62 | 24.74±1.56 | 25.43±0.96 | 23.46±2.84 | |
Sacrificed | 14.0 ±1.4 |
14.1 ±1.1 |
13.4 ±1.1 |
37.21±0.51 | 37.66±0.42 | 37.60±0.36 | ||||
Day 5 |
Pre-operation | 15±0 | 15±0 | 15±0 | 24.32±1.54 | 25.28±1.56 | 22.82±0.84 | 24.76±1.20 | 25.32±1.75 | 23.42±0.76 |
Injection | 14±0 | 13.6 ±0.5 |
13.8 ±0.4 |
37.74±0.18 | 37.48±0.59 | 37.32±0.78 | 26.30±1.72 | 25.82±2.04 | 25.14±1.69 | |
Sacrificed | 14.4 ±0.9 |
15±0 | 14.6 ±0.5 |
37.42±0.55 | 37.72±0.36 | 37.60±0.37 | ||||
Day 7 |
Pre-operation | 15±0 | 15±0 | 15±0 | 25.06±1.61 | 25.22±1.37 | 22.82±0.80 | 23.74±1.74 | 24.00±1.42 | 22.78±0.67 |
Injection | 11.0 ±2.4 |
11.4 ±2.1 |
12.0 ±0.1 |
37.48±0.68 | 38.42±0.54 | 38.08±0.41 | 24.26±2.09 | 24.26±1.89 | 23.78±1.05 | |
Sacrificed | 14.8 ±0.4 |
14.2 ±0.4 |
14.6 ±0.6 |
36.78±0.69 | 38.00±0.37 | 37.16±0.54 |
There was no significant difference between animals injected with PBS and MNCs or RACs, injected on either day 1 (PBS: n = 13, MNCs: n = 12, RACs: n = 13), day 3 (PBS: n = 8, MNCs: n = 8, RACs: n = 7), day 5 (each group: n = 5), or day 7 (each group: n = 5) in terms of Bederson score at the late phase, weight at any time, and temperature at any time (pre-operation, injection, and scarification).