Table 1. Relative quantification and comparison of the PG muropeptides of S. epidermidis ST10002 (PIA+) and AZ39 (PIA-) biofilm cells grown in TSBg and PCs.
| Peak Number | Chemical composition | AZ39 | Peak Number | Chemical composition | ST10002 | ||
|---|---|---|---|---|---|---|---|
| TSBg | PC | TSBg | PC | ||||
| % Corr area | % Corr area | % Corr area | % Corr area | ||||
| 1, 5 | NAG-NAM-A-Q-K-A-A | 7.05 | 41.48 | 1, 5, 6 | NAG-NAM-A-Q-K-A-A | 9.87 | 34.48 |
| 2 | NAG-NAM-A-Q-K-(G)-A-A | 5.67 | 6.52 | 2 | NAG-NAM-A-Q-K-(G)-A-A | 5.99 | 9.57 |
| 2 | NAG-NAM-A-Q-K-(G2S3)-A-A | 0.25 | 0.00 | 2 | NAG-NAM-A-Q-K-(G2S3)-A-A | 0.00 | 0.00 |
| 2 | NAG-NAMOAc-A-Q-K-(S3) | 1.95 | 0.96 | 2 | NAG-NAMOAc-A-Q-K-(S3) | 0.81 | 1.41 |
| 3 | NAG-NAM-A-Q-K-(G4S)-A | 0.66 | 0.00 | 3 | NAG-NAM-A-Q-K-(G-G-G-G-G)-A | 2.69 | 0.11 |
| 4 | NAG-NAM-A-Q-K-(G-G)-A-A | 2.46 | 1.62 | 4 | NAG-NAM-A-Q-K-(G-G)-A-A | 1.49 | 3.98 |
| 4 | NAG-NAM-A-Q-K-(G-G-G)-A-A | 0.26 | 0.00 | 4,5 | NAG-NAM-A-Q-K-(G-G-G)-A-A | 0.52 | 0.97 |
| 4 | NAG-NAM-A-Q-K-(G3S2)-A-A | 0.00 | 1.11 | 4,6 | NAG-NAM-A-Q-K-(G2S)-A-A | 0.00 | 0.84 |
| 4,6 | NAG-NAM-A-Q-K-(G2S)-A-A | 2.25 | 2.84 | 4,15 | NAG-NAMOAc-A-Q-K-(G3-S2)-A-A | 0.63 | 1.34 |
| 5, 6, 7, 20 | NAG-NAMOAc-A-Q-K-A-A | 4.47 | 14.07 | 5 | NAG-NAM-A-Q-K-(G-G-G-G)-A-A | 0.22 | 0.25 |
| 6 | NAG-NAM-A-Q-K-(S3) | 1.55 | 0.00 | 5, 6, 7, 20 | NAG-NAMOAc-A-Q-K-A-A | 7.48 | 4.30 |
| 6, 7, 9 | NAG-NAM-A-Q-K-(G-G-G-G-G)-A-A | 7.16 | 3.81 | 6, 7, 9 | NAG-NAM-A-Q-K-(G-G-G-G-G)-A-A | 5.08 | 3.20 |
| 7 | NAG-NAM-A-Q-K-(G-G-S-G-G)-A-A | 13.97 | 3.95 | 7 | NAG-NAM-A-Q-K-(G-G-S-G-G)-A-A | 0.00 | 0.00 |
| 8 | NAG-NAM-A-Q-K-(A)-A-A | 14.84 | 18.33 | 8 | NAG-NAM-A-Q-K-(A)-A-A | 22.5 | 31.60 |
| 11 | NAG-NAMOAc-A-Q-K-(G-G-G-G-G) | 3.54 | 1.48 | 11 | NAG-NAMOAc-A-Q-K-(G-G-G-G-G) | 8.99 | 3.73 |
| 12 | NAG-NAMOAc-A-Q-K-(G-G-G-G-G)-A | 0.00 | 0.71 | 12 | NAG-NAMOAc-A-Q-K-(G-G-G-G-G)-A | 0.89 | 0.95 |
| 12 | NAG-NAMOAc-A-Q-K-(G4-S) | 3.67 | 0.00 | 12 | NAG-NAMOAc-A-Q-K-(G4-S) | 1.27 | 0.00 |
| 12 | NAG-NAM-A-Q-K-(G4A)-A-A | 2.57 | 0.18 | 12 | NAG-NAM-A-Q-K-(G4A)-A-A | 0.00 | 0.00 |
| 13 | NAG-NAM-A-Q-K-(G3AS)-A-A | 7.49 | 0.00 | 13 | NAG-NAM-A-Q-K-(G3AS)-A-A | 2.85 | 0.00 |
| 14 | NAG-NAMOAc-A-Q-K-(G)-A-A | 0.28 | 0.00 | 15 | NAG-NAMOAc-A-Q-K-(G)-A-A | 0.93 | 0.00 |
| 14 | NAG-NAMOAc-A-Q-K-(G2-S)-A | 0.17 | 0.00 | 15 | NAG-NAMOAc-A-Q-K-(G2-S)-A | 0.55 | 0.00 |
| 14 | NAG-NAMOAc-A-Q-K-(G3-S2)-A-A | 0.19 | 0.00 | 15, 18, 19, 20 | NAG-NAMOAc-A-Q-K-(G4-S)-A-A | 6.07 | 0.00 |
| 14, 17, 19, 20 | NAG-NAMOAc-A-Q-K-(G4-S)-A-A | 7.64 | 0.13 | 18 | NAG-NAMOAc-A-Q-K-(G2S)-A-A | 0.00 | 0.00 |
| 17 | NAG-NAMOAc-A-Q-K-(G-G)-A-A | 0.64 | 0.00 | 18 | NAG-NAMOAc-A-Q-K-(G-G)-A-A | 0.00 | 0.00 |
| 17 | NAG-NAMOAc-A-Q-K-(G2S)-A-A | 0.40 | 0.00 | 19 | NAG-NAMOAc-A-Q-K-(G-G-G-G-G)-A-A | 0.73 | 0.00 |
| 20 | NAG-NAMOAc-A-Q-K-(G-G-G-G-G)-A-A | 0.85 | 0.00 | 19 | NAG-NAMOAc-A-Q-K-(S3) | 1.67 | 0.00 |
| 21, 22, 23 | NAG-NAMOAc-A-Q-K-(A)-A-A | 10.05 | 2.84 | 20, 21, 22 | NAG-NAMOAc-A-Q-K-(A)-A-A | 18.78 | 3.27 |
| TOTAL (%) | 100.02 | 100.03 | TOTAL (%) | 100.01 | 100 | ||
| PG Modifications |
AZ39 | TSBg | PC | PG Modifications |
ST10002 | TSBg | PC |
| Serine muropeptides | 50.30 | 31.66 | Serine muropeptides | 13.85 | 3.59 | ||
| O-acetilation | 37.48 | 5.34 | O-acetilation | 47.99 | 13.59 | ||
| Non A-A muropeptides | 38.61 | 22.41 | Non A-A muropeptides | 16.87 | 6.2 | ||
| S-link | 40.38 | 26.09 | S-link | 29.20 | 9.33 | ||
% Corr area = corresponding area under a peak