Fig 1. Incubation at HHP promotes solubilization of NS1-IBs.

Suspensions of NS1-IBs were subjected to 2.4 kbar for 90 min and to 0.4 kbar for 14 h30 min (2.4/0.4 kbar) or to 1 bar for 16 h. A, LS vs pH; B, LS vs GdnHCl concentration at pH 8.5. The LS value, obtained for the suspension at a pH 7.0 that was not subjected to HHP and read immediately after dilution, was considered to be 100%. C, LS vs pH and presence of Arg. The LS value obtained for the suspension at pH 7.0, determined after 16 h incubation, was considered to be 100%. The LS measurements were carried out in a spectrofluorimeter with an excitation of 320 nm; the emission was determined between 315 and 325 nm and the areas of the peaks were used for plotting. Values are expressed as LS mean ± SD. Each condition was analysed in quadruplicate. The results shown are representative of 3 independent assays.