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SEC of supernatants of DENV NS1-IB suspensions subjected to 2.4 kbar/0.4 kbar. A volume of 500 μl of the supernatants of the suspensions subjected to HHP was applied to a Superdex 200 10/300 column (GE Biosciences). The elution buffer was 50 mM CAPS at a pH of 11.0. The results shown are representative of 3 independent assays.
