Fig 5. NS1 solubilized by application of HHP at pH of 10.0–11.5 and dialyzed at a pH of 8.5 forms mostly trimers, dimers and monomers.

DENV NS1-IB suspensions were subjected to 2.4 kbar/0.4 kbar and dialyzed against 50 mM Tris HCl at a pH of 8.5. A volume of 500 μl of the supernatant of the suspensions was applied to a Superdex 200 10/300 column (GE Biosciences). The elution buffer was TrisHCl 50 mM at a pH of 8.5. The results shown are the representative of 2 independent assays.