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(A) Colocalization in TUNEL (apoptosis, green) and 4′,6-diamidino-2-phenylindole (DAPI; nuclei, blue).(B)Number of TUNEL-positive cells significantly decreased in both IR-HCA and HCA-IR groups. (C and D) Effects of HCA on the expression of the caspase-3 in the five groups. The results are mean ± SEM of three independent experiments. ***P < 0.001, **P < 0.01 versus control group; ###P < 0.001, ##P < 0.01, #P < 0.05 versus IR group.
