Figure 2.

Follicle development in cultured ovaries, cultured reaggregated ovaries, transplanted reaggregated ovaries and age-matched in vivo ovaries. Follicle development (follicle stages and total number of developing follicles) in P2 or P6 reaggregated ovaries (ROs) which have been transplanted for (A) 14 days (P2-ROt + 14d and P6-ROt + 14d) and (B) 21 days (P2-ROt + 21d and P6-ROt + 21d) was assessed. Representative images of (C) ROs generated from P2 and P6 ovaries after 14 days of transplantation, (D) ROs and ovaries prior to culture and (E) after 14 days of culture and (F) in vivo Control ovaries. (G) Total number of follicles in P2 ovaries (P2-Oc + 14d) and P2 ROs (P2-ROc + 14d) cultured for 14 days, P2 ROs transplanted for 14 days (P2-ROt + 14d) and the age-matched in vivo control of P16 ovaries (P16-O) and (H) their equivalent in P6 ovaries and ROs (P6 + Oc + 14d, P6-ROc + 14d, P6-ROt + 14d, P20-O). The total number of follicles for the cultured and transplanted ROs includes putative primordial follicles. (I and J) Follicle development was assessed within these matched P2 and P6 groups. 1°, primary follicles; 2°, secondary follicles; PA, preantral follicles. Scale bar: 500 µm. Data are plotted as mean values ± s.d. P2-Oc + 14d n = 3; P2-ROc + 14d n = 5; P2-ROt + 14d n = 3; P2-ROt + 21d n = 3; P16-O n = 4; P6-Oc + 14d n = 3; P6-ROc + 14d n = 4; P6-ROt + 14d n = 3; P6-ROt + 21d n = 3; P20-O n = 3. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.