Figure 4.

Representative images of follicles at primary, secondary and preantral stages within cultured ovaries, cultured reaggregated ovaries, transplanted reaggregated ovaries and age-matched in vivo ovaries. Representative images of a (A) primary follicle from age-matched in vivo ovaries, cultured ovaries, transplanted reaggregated ovaries (ROs) and cultured ROs. Red box is magnified in A′. (A′) Higher magnification of the red box in A, showing the theca layer of primary follicles. Red arrows are pointing at the theca layer. Representative images of (B) secondary follicles, (B′) higher magnification of secondary follicle theca layers, (C) preantral follicles and (C′) higher magnification of preantral follicle theca layers are shown. Follicle scale bar (A, B and C): 50 µm; high magnification theca scale bar (A′, B′ and C′): 10 µm. #, no follicles present.