Figure 6.

Theca and granulosa cell quantification in ovaries and reaggregated ovaries. Quantification of (A) theca cell number was determined for primary, secondary and preantral follicles in P2 ovaries (P2-O), P2 cultured ovaries (P2-Oc + 14d), P2 cultured reaggregated ovaries (P2-ROc + 14d), P2 transplanted ROs (P2-ROt + 14d) and P16 ovaries (P16-O), as well as for the P6 counterparts (P6-O, P6-Oc + 14d, P6-ROc + 14d, P6-ROt + 14d, P20-O). (B) Granulosa cell number was also determined in primary, secondary and preantral follicles. Data are plotted as mean values ± s.d. Bars with matched lower-case letters are significantly different from each other, within a follicle stage. ^#P2-O and P6-O are not compared to the other conditions, as they have not been cultured for 14 days. Primary follicles: P2-O n = 49; P2-Oc + 14d n = 28; P2-ROc + 14d n = 109; P2-ROt + 14d n = 31; P16-O n = 62; P6-O n = 57; P6-Oc + 14d n = 57; P6-ROc + 14d n = 25; P6-ROt + 14d n = 44; P20-O n = 52. Secondary follicles: P2-Oc + 14d n = 12; P2-ROc + 14d n = 81; P16-O n = 54; P6-O n = 9; P6-Oc + 14d n = 14; P6-ROc + 14d n = 23; P6-ROt + 14d n = 4; P20-O n = 31. Preantral follicles: P2-Oc + 14d n = 5; P2-ROc + 14d n = 8; P16-O n = 19; P6-Oc + 14d n = 11; P6-ROc + 14d n = 13; P20-O n = 19.