Fig 3. Effect of benznidazole and nifurtimox on axenic amastigotes and 3T3 intracellular amastigotes.

Indicated concentration of benznidazole (A) or nifurtimox (B) was added to the culture of axenic amastigotes (closed circle) or 3T3 intracellular amastigotes (open circle). Growth of axenic culture was quantitated by resazurin assay. Relative inhibition was calculated from Relative Fluorescent Units and normalized to no drug control. Growth of intracellular amastigotes in the host 3T3 cells was calculated from the ratio of infected over uninfected host 3T3 cells and normalized to no drug control. Experiment was performed in triplicate, and mean values (±SD) were plotted.