Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 25;14(1):e0211235. doi: 10.1371/journal.pone.0211235

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Tombak et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

Fig 1 — U2OS cells were transfected with 5 μg of MfPV1 (lanes 1–3) and 4 μg of MfPV5 (lanes 7–9) and 8 (12–14) minicircle genomes. Low molecular weight (LMW) DNA was extracted at the indicated time points (days) and analyzed through 1D gel electrophoresis, followed by Southern blotting. Prior to analysis, the samples were treated with DpnI and specific linearizing endonuclease (SdaI for MfPV1, BamHI for MfPV5, and BglI for MfPV8) (Thermo Scientific). Lanes 4, 10 and 15 are size markers for linear MfPV1, 5 and 8 DNA, respectively. Lanes 5, 6 and 11 are size markers for the DpnI fragments of MfPV1, 5 and 8 genomes, respectively. DpnI, the fragments of DpnI-digested unreplicated genomes. Arrow marks the linear MfPV genomes.