Table 1. Nucleotide sequences, amplification sites, GenBank accession numbers, amplimer sizes and PCR reaction conditions for each primer pair are shown.
| Gene | Primer | Nucleotide sequence (5’→3’) | GenBank ID | Annealing temperature | Amplimer size (bp) |
|---|---|---|---|---|---|
| antisense | GCT GTA TTG CTC CTC CCT (518–535) |
||||
|
Actin (Actb) |
sense |
GCC AAC CGT GAA AAG ATG A (419–437) |
NM_007393.5 | 54°C | 462 |
| antisense | CAA GAA GGA AGG CTG GAA AA (861–880) |
||||
| PAC1 (ADCYAP1R1) | sense | TAT TAC TAC CTG TCG GTG AAG (912–932) |
NM_016989.2 | 49°C | 213 |
| antisense | ATG ACT GCT GTC CTG CTC (1107–1124) |
||||
|
VPAC1 (VIPR1) |
sense | TTT GAG GAT TTC GGG TGC (974–991) |
NM_001097523 | 52°C | 266 |
| antisense | TGG GCC TTA AAG TTG TCG (1222–1239) |
||||
|
VPAC2 (VIPR2) |
sense | CTC CTG GTA GCC ATC CTT (805–822) |
NM_001014970 | 48°C | 149 |
| antisense | ATG CTG TGG TCG TTT GTG (936–953) |