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. Author manuscript; available in PMC: 2019 Jan 25.
Published in final edited form as: Cell Rep. 2019 Jan 2;26(1):29–36.e3. doi: 10.1016/j.celrep.2018.12.037

Figure 1. Cryo-EM Imaging of a Catalytically Inactive Hsp104 Variant.

Figure 1.

(A) ATPase activities of Hsp104 and Hsp104DWB without and with κ-casein stimulation. Averages of three independent measurements ± SD are shown.

(B and C) Determining the nucleotide occupancy in Hsp104DWB using (B) MANT-ATP and (C) BzATP crosslinking, respectively. Average number of bound nucleotide from at least three independent measurements ± SD is shown.

(D) Representative EM micrograph of Hsp104DWB hexamers embedded in vitreous ice (scale bar, 25 nm).

(E) Images of selected 2D classes from reference-free 2D classification.