KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
E. coli BL21-CodonPlus (DE3)-RIL	Stratagene	N/A
Chemicals, Peptides, and Recombinant Proteins
ATP	Roche	Cat.#10127531001; CAS: 51963-61-2
MANT-ATP	Jena Bioscience	Cat.#NU-202; CAS: 151481-86-6
BzATP	Sigma-Aldrich	Cat.#B6396; CAS: 112898-15-4
Malachite green	Sigma-Aldrich	Cat.#M9636; CAS: 123333-61-9
Ammonium molybdate	Sigma-Aldrich	Cat.#A1343; CAS: 12054-85-2
Citric acid	Sigma-Aldrich	Cat.#C7129; CAS: 5949-29-1
Ni-NTA agarose	QIAGEN	Cat.#30230
Q-Sepharose Fast Flow	GE Healthcare	Cat.#17051001
Superdex 200 10/300 GL column	GE Healthcare	Cat.#17108801
Zeba Spin desalting column	Thermo Fisher	Cat.#89882
PD-10 column	GE Healthcare	Cat.#17085101
κ-casein	Sigma-Aldrich	Cat.#C0406; CAS: 9000-71-9
Deposited Data
Hsp104DWB (Open)	This paper	PDB: 6N8V; EMD-0376
Hsp104DWB (Closed)	This paper	PDB: 6N8T; EMD-0375
Hsp104DWB (Extended)	This paper	PDB: 6N8Z; EMD-0377
Recombinant DNA
Plasmid expressing Hsp104	Lee et. al, 2010	pHsp104
Plasmid expressing Hsp104DWB	Lee et. al, 2010	pTRAP
Software and Algorithms
Microsoft Excel	Microsoft	https://products.office.com/en-us/excel
SerialEM	Mastronarde, 2005	http://bio3d.colorado.edu/SerialEM/
UNBLUR	Grant and Grigorieff, 2015	http://grigoriefflab.janelia.org/unblur
EMAN2	Bell etal., 2016	https://blake.bcm.edu/emanwiki/EMAN2
RELION 1.4	Scheres, 2012	http://www2.mrc-lmb.cam.ac.uk/relion
Chimera	Pettersen et al., 2004	https://www.cgl.ucsf.edu/chimera
Other
Holey carbon grid, Cu 400 mesh	Quantifoil Micro Tools GmbH	Quantifoil R 1.2/1.3