PCL/Coll nanofibers with TGF-β1 support the attachment and fibroblast-to-myofibroblast differentiation of NHDFs. Immunofluorescent staining of focal adhesion protein vinculin (green) and nuclei with DAPI (blue) (A,B), and immunofluorescent staining of FAK (green) (C,D), and α-SMA (red) (E, F) after 7 days culture of 2×104 cells/sample. (G) Cell migration tracks, with individual cell tracks displayed in different colors. Each track start was equalized to the center of the plot. Plot is shown from −150 to 150 μm. (H) Mean displacement for the NHDFs (n=10) tracked at 60-min lapse interval. (I) Quantification of α-SMA positive cells normalized against the cell nuclei stained with DAPI. Data presented as mean ± SD, n=3, p-values are calculated using an unpaired student t-test, **p <0.01.