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. 2019 Jan 25;14(1):e0211012. doi: 10.1371/journal.pone.0211012

Fig 1. Characterization of FDG labeling of human erythrocytes.

Fig 1

(A) FDG uptake by 5 day old and 1 day old human erythrocytes.The relative percent FDG uptake by 5 days old human erythrocytes (post-phlebotomy) vs.1 day old human erythrocytes. Either 250 μl of 1 day old human erythrocytes or 5 day old erythrocytes were incubated with 100 μl (≈ 37MBq) 18F-FDG for 2 hours at 37° C. (B) Human erythrocyte uptake of FDG after 30 minute incubation approaches that of 2 hour incubation. 250 μl of 1 day old human erythrocytes were incubated with 100 μl (≈ 37MBq) FDG at 37° C for 30 minutes, 1 hour, and 2 hours. Sample number = 3/timepoint. (C) Relative % free FDG remaining in the FDG-labeled erythrocyte fraction (250 μl), original incubation supernatant, and wash supernatants. (D) Slow gradual release of intracellular FDG over time (R2 = 0.9586; sample number = 3/timepoint) (S1 File).