Table 6. Mean scores for the different histopathological parameters used to assess the kidney, spleen and liver damage in Atlantic salmon (Salmo salar L.) exposed to graded levels of ethoxyquin (EQ) through their diet for 90 day.
| Dietary treatment | ||||||
|---|---|---|---|---|---|---|
| Parameter assessed | EQ 0 | EQ 1 | EQ 2 | EQ 3 | EQ 4 | EQ 5 |
| Kidney | ||||||
| Presence of pigmented macrophages aggregates (PMA) | 0.3 ± 0.3 | 0.2 ± 0.2 | 0.2 ± 0.2 | 0.4 ± 0.4 | 0.4 ± 0.4 | 1.2 ± 1.2* |
| Spleen | ||||||
| Congestion and subcapsular hemorrhages | 0.7 ± 0.7 | 0.2 ± 0.3 | 0.1 ± 0.2 | 1.0 ± 0.6 | 1.0 ± 0.5 | 1.6 ± 0.5* |
| Liver | ||||||
| Vacuolization in hepatocyte cytoplasm | 2.1 ± 0.6 | 2.1 ± 0.6 | 2.2 ± 0.8 | 0.7 ± 0.4* | 0.5 ± 0.3* | 0.2 ± 0.2* |
Scoring was performed in a double-blinded format. Results are presented as mean ± SD of 9 individually scored animals per experimental group.
Group mean scores were compared using Pearson’s X2 (level of confidence 95%).
* p<0.05. compared to the control (EQ 0).