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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Cell Signal. 2018 Oct 5;53:140–150. doi: 10.1016/j.cellsig.2018.10.003

Figure 4: Intracellular Ca2+ in PN vs. PH cells.

Figure 4:

A) Representative traces of intracellular Ca2+ release in response to Thapsigargin (TG) in PN or PH cells. Cells were kept in zero Ca2+ for approximately one hour prior to the imaging experiment to induce SOCE and subsequently treated with 5 mM Ca2+ for the time indicated. Intracellular Ca2+ (F340/F380) levels obtained by ratiometric Fura-2 AM analysis of PN/PH cells. B) Graph summarizes resting calcium levels. C) Summarizes the average spike amplitude of Ca2+ release in response to TG in absence of extracellular calcium. D) Summarizes the average spike amplitude of Ca2+ release in response to 5 mM extracellular Ca2+ in presence of TG. Amplitude was measured as standard deviation of signal base to peak fluorescence ratio. Asterisk indicates significance between the two groups. *P < 0.05 **P < 0.01, ****P < 0.0001 (n=4-5).