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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Transplantation. 2019 Feb;103(2):307–313. doi: 10.1097/TP.0000000000002455

Figure 2: Pathways of Regeneration (DNA Synthesis).

Figure 2:

Human renal biopsies were taken following 24 hours of Exsanguinous Metabolic Support with or without the addition of 1×108 mesenchymal stem cells. Tissue samples were fixed in 4% formaldehyde, processed and embedded in paraffin wax for sectioning. An indirect immunofluorescent assay was performed using a Mouse Anti-PCNA antibody (ABCAM) and Goat Anti-Mouse Alexa Fluor 488 secondary. A.) Represents PCNA expression after 24H of EMS perfusion B.) MSC treated human kidneys resulted in a significant increase in PCNA positive nuclei in the paired test kidneys (p<0.049) (n=5 pairs).