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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Curr Protoc Toxicol. 2018 Nov 2;79(1):e66. doi: 10.1002/cptx.66

Table 2:

List of fluorescent dyes and their concentrations used in this protocol

Fluorescent dyes Assays/endpoints Working stock conc. (mM) Working conc. (μM) Working solvent
Hoechst 33342 DNA damage 10 25 PBS
Tetramethyl rhodamine methyl ester (TMRM) MMP/ Mitochondrial impairment 0.5 0.5 PBS
Monochlorobimane (mBCl) Intracellular glutathione level/oxidative stress 200 100 PBS
Calcein AM Membrane integrity/cell viability 1 0.25 PBS