Table 2:
List of fluorescent dyes and their concentrations used in this protocol
| Fluorescent dyes | Assays/endpoints | Working stock conc. (mM) | Working conc. (μM) | Working solvent |
|---|---|---|---|---|
| Hoechst 33342 | DNA damage | 10 | 25 | PBS |
| Tetramethyl rhodamine methyl ester (TMRM) | MMP/ Mitochondrial impairment | 0.5 | 0.5 | PBS |
| Monochlorobimane (mBCl) | Intracellular glutathione level/oxidative stress | 200 | 100 | PBS |
| Calcein AM | Membrane integrity/cell viability | 1 | 0.25 | PBS |