Fig. 4.

Temporal control over nucleation. a Schematic representation of delay in growth through ligand dilution and delayed activation. Ligand dilution represents ethanol amine (EA) acting as a competitive inhibitor. b Schematic representation of delay in growth through delayed pH change using urease. c Absorbance trend, at 515 nm, of 1.2-8A growth with various equivalents of EA (inset: same graph on linear scale to easily elucidate the change in lag phase). ([1] = [2] = [8A] = 1 mM, 50 mM pH = 11 buffer). d Absorbance trend, at 515 nm, of 1.2-8A growth with variation of urease concentration ([Urea] = 400 mM, [1] = [2] = 1 mM, [8A] = 5 mM, 200 mM pH = 7 buffer)