Table 2.

KpnI RFLP analysis of mESC clones.

Cas9	ssODN	MMC	Analyzed number of clones	Positive KpnI RFLP%	KpnI homo/hemizygosity%
WT	Sense	−	47	10.6	4.3
	Sense	+	47	12.8	4.3
	AS	−	45	20.0	2.2
	AS	+	47	53.2	12.8
D10A	Sense	−	48	10.4	2.1
	Sense	+	5	80.0	0
	AS	−	48	31.2	0
	AS	+	22	81.8	0