Fig. 4. SETD3-positively regulates the activation of DNA-damage-induced apoptosis target genes.

a Immunoprecipitation of endogenous SETD3 from whole-cell lysate (HCT-116 cells) followed by western blot with the indicated antibodies. b ELISA experiment between His-p53 and His-SUMO SETD3, His-SUMO FoxM1 used as positive control. c In vitro methylation assay between His-SUMO SETD3 and His-p53. Using His-SUMO FoxM1 as positive control. d qPCR analysis of extracted mRNA from HCT-116 control and KO cells, as indicated, post DOX or vehicle treatment. Graphs show the relative expression levels (normalized to GAPDH) of the indicated genes. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001