Table 1.
Phylogenetic affiliation and origin of the Sph isolates
| Strain (clade) | Accession numbera | Closest relative (% identity) | Closest cultivated relative (% identity) | Membrane typeb | Feed water |
|---|---|---|---|---|---|
| Sph1 (A) | KP866793 | Sphingomonas sp. GW5 (100%) | Sphingomonas parapaucimobilis strain NBRC 15100 (99%) | RO | Surface water (A.G. Wildervanckkanaal) |
| Sph2 (B) | KP866794 | Sphingomonas pseudosanguinis strain HPLM-2 (100%) | Sphingomonas sanguinis strain NBRC 13937 (99%) | RO | Industrial wastewater (Starch production) |
| Sph3 (B) | KP866795 | Sphingomonas pseudosanguinis strain HPLM-2 (99%) | Sphingomonas sanguinis strain NBRC 13937 (99%) | RO | Industrial wastewater (Starch production) |
| Sph4 (C) | KP866796 | Sphingobium sp. EMB 221 (99%) | Sphingobium yanoikuyae strain NBRC 15102 (99%) | NF | Municipal wastewater |
| Sph5 (D) | KP866797 | Sphingomonas sanguinis strain BAB-7166 (99%) | Sphingomonas sanguinis strain NBRC 13937 (99%) | MF | Tap water |
| Sph6 (E) | KP866798 | Sphingomonas echinoides strain NRRL B-3127 (100%) | Sphingomonas echinoides strain NBRC 15742 (99%) | ROc | Tap water |
| Sph7 (E) | KP866799 | Sphingomonas echinoides strain NRRL B-3127 (100%) | Sphingomonas echinoides strain NBRC 15742 (99%) | ROc | Tap water |
| Sph10 (F) | KP866800 | Sphingomonas sp. ZJ116 (100%) | Sphingomonas melonis strain DAPP-PG 224 (100%) | RO | Industrial wastewater (Starch production) |
| Sph11 (G) | KP866801 | Sphingomonas sp. V1-2 (99%) | Sphingomonas hankookensis strain ODN7 (99%) | RO | Industrial wastewater (Starch production) |
| Sph16 (H) | KP866802 | Uncultured bacterium clone HK34-1-11-4 (100%) | Sphingomonas aquatilis strain NBRC 16722 (98%) | RO | Surface water (A.G. Wildervanckkanaal) |
| Sph19 (A) | KP866803 | Sphingomonas sp. GW5 (99%) | Sphingomonas parapaucimobilis strain NBRC 15100 (99%) | RO | Surface water (A.G. Wildervanckkanaal) |
| Sph22 (I) | KP866804 | Uncultured bacterium HOClCi53 (100%) | Sphingomonas hankookensis strain ODN7 (99%) | RO | Surface water (A.G. Wildervanckkanaal) |
| Sph25 (A) | KP866805 | Sphingomonas sp. GW5 (100%) | Sphingomonas parapaucimobilis strain NBRC 15100 (99%) | RO | Surface water (A.G. Wildervanckkanaal) |
| Sph27 (J) | KP866806 | Sphingomonas sp. 2R-2 (99%) | Sphingomonas hankookensis strain ODN7 (99%) | RO | Industrial wastewater (Starch production) |
| Sph29 (A) | KP866807 | Sphingomonas sp. GW5 (99%) | Sphingomonas parapaucimobilis strain NBRC 15100 (99%) | RO | Industrial wastewater (Starch production) |
| Sph30 (A) | KP866808 | Sphingomonas sp. GW5 (99%) | Sphingomonas parapaucimobilis strain NBRC 15100 (99%) | RO | Industrial wastewater (Starch production) |
| Sph31 (A) | KP866809 | Sphingomonas sp. GW5 (100%) | Sphingomonas parapaucimobilis strain NBRC 15100 (99%) | RO | Industrial wastewater (Starch production) |
| Sph32 (A) | KP866810 | Sphingomonas sp. GW5 (100%) | Sphingomonas parapaucimobilis strain NBRC 15100 (99%) | RO | Industrial wastewater (Starch production) |
| Sph33 (K) | KP866811 | Sphingopyxis macrogoltabida strain BSN54 (100%) | Sphingopyxis soli strain BL03 (99%) | RO | Industrial wastewater (Starch production) |
| Sph46 (L) | KP866812 | Sphingomonas sp. HX-H01 (99%) | Sphingobium xenophagum strain BN6 (99%) | NF | Anoxic groundwater |
| Sph57 (L) | KP866813 | Sphingomonas sp. HX-H01 (99%) | Sphingobium xenophagum strain BN6 (99%) | NF | Anoxic groundwater |
aThe 16S rRNA sequences of the Sph isolates have been deposited in Genbank under the accession numbers KP866793 - KP866813
bRO = reverse osmosis, NF = nanofiltration, MF = microfiltration
cBacteria isolated from membranes used in laboratory experiments