Table 2.

Physiological characteristics of the Sph isolates

Strain (clade)	Swimming		Swarming	Twitching		Growth ranges
	Microa	Macrob		Microc	Macrod	pH	Temp (°C)	Salt(NaCl% w/v)
Sph1 (A)	+	-	-	+	+	5.0–10.0	8–37	0–5.0
Sph2 (B)	+	+	-	+	+	5.0–10.0	8–37	0–5.0
Sph3 (B)	+	-	-	+	+	5.0–10.0	8–37	0–5.0
Sph4 (C)	+	+	-	+	-	5.0–10.0	8–37	0–3.5
Sph5 (D)	+	-	+	+	+	5.0–10.0	8–37	0–5.0
Sph6 (E)	+	+	+	+	+	5.0–10.0	8–37	0–5.0
Sph7 (E)	+	+	-	+	-	4.0–9.0	8–37	0–3.5
Sph10 (F)	-	+	-	+	-	5.0–10.0	8–37	0–3.5
Sph11 (G)	+	+	+	+	+	6.0–10.0	8–37	0–3.5
Sph16 (H)	-	-	-	+	+	4.0–10.0	8–42	0–3.5
Sph19 (A)	+	+	+	+	+	5.0–9.0	8–37	0–5.0
Sph22 (I)	+	+	-	+	+	5.0–10.0	8–37	0–3.5
Sph25 (A)	+	-	+	+	-	5.0–9.0	8–37	0–5.0
Sph27 (J)	+	+	-	+	+	5.0–9.0	8–37	0–3.5
Sph29 (A)	+	+	-	+	+	5.0–9.0	8–37	0–5.0
Sph30 (A)	+	+	+	+	+	5.0–10.0	8–37	0–5.0
Sph31 (A)	+	+	+	+	+	5.0–9.0	8–37	0–5.0
Sph32 (A)	+	+	+	+	+	5.0–10.0	8–37	0–5.0
Sph33 (K)	-	-	-	+	-	4.0–10.0	8–37	0–3.5
Sph46 (L)	+	-	-	+	+	5.0–10.0	8–37	0–3.5
Sph57 (L)	+	-	-	+	+	5.0–10.0	8–42	0–3.5

^aSwimming was assayed microscopically by phase contrast microscopy

^bSwimming and swarming was assayed macroscopically by plate assays

^cTwitching was assayed microscopically by growing the strains on TMGG medium amidst of a microscopic slide and a glass coverslip

^dTwitching was assayed macroscopically by growing the cells on twitching plates